CDC42BPB antibody

Catalog No. ABIN498363

The Rabbit Polyclonal anti-CDC42BPB antibody (ABIN498363) specifically detects CDC42BPB in WB and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CDC42BPB antibody (ABIN498363)

Target: CDC42BPB (CDC42 Binding Protein Kinase beta (DMPK-Like) (CDC42BPB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42BPB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC42BPB Antibody

Specificity: This antibody detects endogenous levels of MRCKbeta protein. (region surrounding Phe1665)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDC42BPB

Target: CDC42BPB (CDC42 Binding Protein Kinase beta (DMPK-Like) (CDC42BPB))

Alternative Name: CDC42BPB

Background: Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Myotonic dystrophy kinaserelated Cdc42-binding (DMPK-like) kinases-alpha and beta (MRCK-alpha, beta) contain a cysteine-rich motif and a putative pleckstrin homology domain. MRCKs can phosphorylate nonmuscle Myosin light chain and influences Actin-Myosin contractility. MRCK-alpha can phosphorylate and activate LIM kinases downstream of Cdc42, which leads to inactivation of ADF/Cofilin and to Actin cytoskeletal reorganization. MRCK-alpha can also influence neurite outgrowth promoted by Cdc42 and Rac.Synonyms: CDC42-binding protein kinase beta, DMPK-like beta, KIAA1124, MRCK beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta, Serine/threonine-protein kinase MRCK beta

Molecular Weight: approx. 194 kDa

Gene ID: 9578

NCBI Accession: NP_006026

UniProt: Q9Y5S2