14-3-3 gamma antibody

Catalog No. ABIN498437

Product Details anti-14-3-3 gamma Antibody

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of 14-3-3 gamma protein. (region surrounding Met81)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: 14-3-3 Protein gamma (YWHAG1 Products)

Synonyms: 14-3-3GAMMA antibody, YWHAG antibody, SRCRB4D antibody, 14-3-3g antibody, 14-3-3gamma antibody, ywhag antibody, 14-3-3G antibody, D7Bwg1348e antibody, cb47 antibody, fd20c02 antibody, wu:fd20c02 antibody, wu:fj53d01 antibody, zgc:73131 antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma L homeolog antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide antibody, 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 antibody, YWHAG antibody, ywhag.L antibody, Ywhag antibody, ywhag1 antibody

Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAG

Molecular Weight: approx. 28 kDa

Gene ID: 7532

NCBI Accession: NP_036611

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase