POLR2E antibody

Catalog No. ABIN498959

The Rabbit Polyclonal anti-POLR2E antibody is suitable to detect POLR2E in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for POLR2E antibody (ABIN498959)

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLR2E Antibody

Specificity: This antibody detects endogenous levels of POLR2E protein. (region surrounding Leu58)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POLR2E

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Alternative Name: POLR2E

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in twofold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.Synonyms: DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, II, RNA polymerases I, RPABC1, RPB5 homolog, XAP4, and III subunit ABC1, and III subunit RPABC1

Molecular Weight: approx. 25 kDa

Gene ID: 5434

NCBI Accession: NP_002686

UniProt: P19388

Pathways: Regulatory RNA Pathways