EBF1 antibody (Internal Region)

Catalog No. ABIN499164

The Goat Polyclonal anti-EBF1 antibody (ABIN499164) specifically detects EBF1 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EBF1 antibody (Internal Region) (ABIN499164)

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This EBF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EBF1 Antibody

Binding Specificity: Internal Region

Sequence: C-HNQLPALANT SV

Specificity: This antibody detects EBF1 / COE1 (Internal region). It is expected not to cross-react with EBF2, EBF3 and EBF4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, ratSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_076870.1

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-0.1 μg/mL, approx.75 kDa band observed in Human Adipose lysates. Anonymous customer used this product successfully in IP from induced 3T3-L1 expressingFLAG-EBF1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for EBF1

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Alternative Name: EBF1 / COE1

Background: EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.Synonyms: EBF, Early B-cell factor, OE-1, Transcription factor COE1

Gene ID: 1879

NCBI Accession: NP_076870

UniProt: Q9UH73