APOBEC3G antibody (N-Term)

Catalog No. ABIN499313

The Rabbit Polyclonal anti-APOBEC3G antibody is suitable to detect APOBEC3G in samples from Human. It has been validated for WB, IHC (p) and EIA.

Quick Overview for APOBEC3G antibody (N-Term) (ABIN499313)

Target: APOBEC3G (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APOBEC3G Antibody

Binding Specificity: N-Term

Specificity: APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G antibody will also detect the APOBEC3F isoform.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human APOBEC3G (N-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: APOBEC3G antibody can be used for detection of APOBEC3G at 5 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for APOBEC3G

Target: APOBEC3G (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G))

Alternative Name: APOBEC3G

Background: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3) as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome (4,5). This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G, a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity (6).Synonyms: APOBEC-3G, APOBEC-related cytidine deaminase, ARCD, ARP9, CEM15, DNA dC->dU-editing enzyme APOBEC-3G, MDS019

Gene ID: 60489

NCBI Accession: NP_068594

UniProt: Q9HC16