CHEK2 antibody (N-Term)

Catalog No. ABIN499619

The Rabbit Polyclonal anti-CHEK2 antibody is suitable to detect CHEK2 in samples from Human, Mouse and Rat. It has been validated for WB, IF and EIA.

Quick Overview for CHEK2 antibody (N-Term) (ABIN499619)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CHEK2 Antibody

Binding Specificity: N-Term

Specificity: Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Chk2 (N-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Chk2 antibody can be used for detection of Chk2 at 0.5 to 1 μg/mL. A 60 kDaband can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2

Background: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2

Gene ID: 11200

NCBI Accession: NP_009125

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle