BAX antibody (AA 84-175) (AbBy Fluor® 750)

Catalog No. ABIN4997215

The Rabbit Polyclonal anti-BAX antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect BAX in samples from Human, Mouse, Rat, Pig, Cow, Rabbit, Chicken and Zebrafish (Danio rerio).

Quick Overview for BAX antibody (AA 84-175) (AbBy Fluor® 750) (ABIN4997215)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat, Pig, Cow, Rabbit, Chicken, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-BAX Antibody (AbBy Fluor® 750)

Binding Specificity: AA 84-175

Cross-Reactivity: Chicken, Cow, Human, Mouse, Pig, Rabbit, Rat, Zebrafish (Danio rerio)

Predicted Reactivity: Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Bax

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background:

Synonyms: BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX

Background: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response