CD45 antibody (AA 1210-1304) (AbBy Fluor® 750)

Catalog No. ABIN4999571

The AbBy Fluor® 750-conjugated Rabbit Polyclonal anti-CD45 antibody (ABIN4999571) specifically detects CD45 in FACS, WB, IF (p) and IF (cc). The antibody is reactive with Human, Mouse, Rat and Rabbit samples.

Quick Overview for CD45 antibody (AA 1210-1304) (AbBy Fluor® 750) (ABIN4999571)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD45 antibody is conjugated to AbBy Fluor® 750

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-CD45 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 1210-1304

Specificity: The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD45

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background:

Synonyms: LCA, LY5, B220, CD45, L-CA, T2, CD45R, GP18, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC

Background: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Gene ID: 5788

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling