ITGA4 antibody (Center)

Catalog No. ABIN500032

This Rabbit Polyclonal antibody specifically detects ITGA4 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for ITGA4 antibody (Center) (ABIN500032)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ITGA4 Antibody

Binding Specificity: Center

Specificity: This antibody reacts to Integrin alpha 4.

Purification: Affinity chromatography purified via peptide column

Immunogen: Integrin alpha 4 antibody was raised against a 15 amino acid peptide from near the center of human Integrin alpha 4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.5 - 1 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-ITGA4 antibody (ABIN500032)

Gąciarz, Ruddock: "Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189964, (2018) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4

Background: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha 4 recombinant protein fragment and thus will recognize it.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 3676

UniProt: P13612

Pathways: Integrin Complex