ELISA, FM, WB
ELISA. Western Blot: 0.5 - 1 μg/mL. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
For Research Use only
PBS containing 0.02 % sodium azide.
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Store the antibody undiluted at 2-8 °C.
Gąciarz, Ruddock: "Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189964, 2018 (PubMed).
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha 4 recombinant protein fragment and thus will recognize it.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4