PRDM16 antibody (C-Term)

Catalog No. ABIN500533

This Rabbit Polyclonal antibody specifically detects PRDM16 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRDM16 antibody (C-Term) (ABIN500533)

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PRDM16 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Peptide Affinity Chromatography

Immunogen: PRDM16 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of Human PRDM16.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1-2 μg/mL. Immunohistochemistry on Paraffin Sections. Positive Control: Rat Brain Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt store the antibody (in aliquots) at -20 °C.

Target Details for PRDM16

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Alternative Name: PRDM16

Background: PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1,3)(p36,q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.Synonyms: KIAA1675, MDS1/EVI1-like gene 1, MEL1, PFM13, PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1

Gene ID: 63976

UniProt: Q9HAZ2

Pathways: Stem Cell Maintenance, Brown Fat Cell Differentiation