SKIL antibody (N-Term)

Catalog No. ABIN500786

Product Details anti-SKIL Antibody

Target: SKIL (SKI-Like Oncogene (SKIL))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects SKIL / SnoN. It will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinita chromatography

Immunogen: SnoN antibody was raised against a 16 amino acid peptide from near the amino terminus of human SnoN.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SKIL / SnoN (SKIL Products)

Synonyms: SNO antibody, SnoA antibody, SnoI antibody, SnoN antibody, Skir antibody, Sno antibody, SKIL antibody, DKFZp459L122 antibody, sno antibody, zgc:193698 antibody, SKI like proto-oncogene antibody, SKI-like proto-oncogene antibody, ski-like protein antibody, SKI-like antibody, SKI-like proto-oncogene b antibody, SKIL antibody, Skil antibody, LOC100010058 antibody, skil antibody, skilb antibody

Background: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN.Synonyms: SNO, Ski-like protein, Ski-related novel protein N, Ski-related oncogene, Ski-related protein, SnoA, Snol

Gene ID: 6498

NCBI Accession: NP_005405

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus