PCK1 antibody (AA 201-300) (AbBy Fluor® 680)

Catalog No. ABIN5008471

The Rabbit Polyclonal anti-PCK1 antibody is suitable to detect PCK1 in samples from Human. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for PCK1 antibody (AA 201-300) (AbBy Fluor® 680) (ABIN5008471)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PCK1 Antibody (AbBy Fluor® 680)

Binding Specificity: AA 201-300

Purpose: PCK1 Polyclonal Antibody, AbBy Fluor-680 Conjugated

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCK1

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background:

Synonyms: PCK1, PEPC, GTP, MGC22652, PEP carboxykinase, PEPCK 1, PEPCK1, PEPCKC, Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase.

Background: PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.

Gene ID: 5105

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis