PP2A alpha/beta antibody (AA 205-309) (Alexa Fluor 750)

Catalog No. ABIN5009068

Product Details anti-PP2A alpha/beta Antibody (Alexa Fluor 750)

Target: PP2A alpha/beta (PP2A A/B)

Binding Specificity: AA 205-309

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PP2A alpha/beta antibody is conjugated to Alexa Fluor 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PP-2A

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PP2A alpha/beta

Target: PP2A alpha/beta (PP2A A/B)

Alternative Name: PP2A alpha / beta (PP2A A/B Products)

Background:

Synonyms: RP-C, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, PPP2CA

Background: PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV4 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Gene ID: 5515

UniProt: P67775