RBPJ antibody (AA 101-200) (AbBy Fluor® 680)

Catalog No. ABIN5009668

The Rabbit Polyclonal anti-RBPJ antibody is suitable to detect RBPJ in samples from Human and Mouse. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for RBPJ antibody (AA 101-200) (AbBy Fluor® 680) (ABIN5009668)

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBPJ antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RBPJ Antibody (AbBy Fluor® 680)

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RBPJK

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RBPJ

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Alternative Name: RBPJK

Background:

Synonyms: SUH, csl, AOS3, CBF1, KBF2, RBP-J, RBPJK, IGKJRB, RBPSUH, IGKJRB1, Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, RBP-JK, Renal carcinoma antigen NY-REN-30, RBPJ

Background: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.

Gene ID: 3516

UniProt: Q06330

Pathways: Notch Signaling, Stem Cell Maintenance, Smooth Muscle Cell Migration