Vimentin antibody

Catalog No. ABIN5012423

The Mouse Monoclonal anti-Vimentin antibody (Clone 3B7) (ABIN5012423) specifically detects Vimentin in WB, IF, FACS, IHC (p), ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Vimentin antibody (ABIN5012423)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Clone: 3B7

Product Details anti-Vimentin Antibody

Purpose: Vimentin Monoclonal Antibody

Specificity: KO-Validated

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: Recombinant human Vimentin Protein

Isotype: IgG

Application Details

Application Notes: WB(1:500-2000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1 μg/Test), ICC/IF(1:50-100)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Synonyms: Vimentin, VIM

Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 Publication Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis