WEE1 antibody (pSer123) (AbBy Fluor® 750)

Catalog No. ABIN5012492

This anti-WEE1 antibody is a Rabbit Polyclonal antibody detecting WEE1 in WB, IF (cc) and IF (p). Suitable for Mouse.

Quick Overview for WEE1 antibody (pSer123) (AbBy Fluor® 750) (ABIN5012492)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-WEE1 Antibody (AbBy Fluor® 750)

Binding Specificity: pSer123

Specificity: This phosphorylation site is homologous to Ser123 in Mouse, Rat, and Bovine. The peptide used to produce this antibody has high homology with a sequence occurring in MIER3, and may react with this protein in Mouse and Human when phosphorylated at the site of Ser10.

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV]

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: Wee1

Background:

Synonyms: WEE1A, WEE1hu, Wee1-like protein kinase, Wee1A kinase, WEE1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Gene ID: 7465

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase