Topoisomerase II alpha antibody (AA 1326-1512)

Catalog No. ABIN5027278

The Mouse Monoclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human. It has been validated for WB and FACS.

Quick Overview for Topoisomerase II alpha antibody (AA 1326-1512) (ABIN5027278)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ABM48B4

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1326-1512

Purification: Protein G Chromatography

Immunogen: A partial length recombinant human Topo II alpha protein (amino acids 1326-1512) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 1-2 μg/mL,FACS Analysis: 0.5-1 μg/10^6 cells

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topo II alpha

Background: Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.

Molecular Weight: 190 kDa

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases