MBD3 antibody (AA 1-290)

Catalog No. ABIN5027348

Product Details anti-MBD3 Antibody

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Binding Specificity: AA 1-290

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification: Protein G Chromatography

Immunogen: A partial length recombinant MBD3 protein (amino acids 1-290) was used as the immunogen for this antibody.

Clone: ABM14A8

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 2-4 μg/mL, IHC: 5 μg/mL, FACS: 0.5 μg/10^6 cells

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD2/MBD3 (MBD3 Products)

Synonyms: xmbd3 antibody, MGC69548 antibody, MBD3 antibody, DKFZp459N1635 antibody, AI181826 antibody, AU019209 antibody, methyl-CpG binding domain protein 3 S homeolog antibody, methyl-CpG binding domain protein 3 antibody, mbd3.S antibody, mbd3 antibody, MBD3 antibody, Mbd3 antibody

Background: MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. This protein binds around 1 kb downstream of the transcription start site of a subset of 400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. Methylated MBD2 is involved in silencing methylated tumor suppressor genes as well as activation of pro-metastatic genes. MBD2 also plays a key role in regulating expression of a range of genes that are associated with optimal DC (Dendritic cells) activation and function.

Molecular Weight: 32. 44 kDa

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding