O-GlcNAc antibody

Catalog No. ABIN5067127

Product Details

Target: O-GlcNAc

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity: Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.

Purification: PEG Purified

Immunogen: Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).

Clone: 9H6

Isotype: IgM

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5067127 was sufficient for detection of O-GlcNAc in 2 μg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: O-GlcNAc

Target Type: Viral Protein