4-Hydroxy-2-Hexenal (4-HHE) antibody (Biotin)

Catalog No. ABIN5067282

Product Details

Target: 4-Hydroxy-2-Hexenal (4-HHE)

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Specific for 4-HHE and 4-HNE modified proteins. Does not detect free 4-HHE or 4-HNE. Does not X-react with Acrolein, Crotonaldehyde, HEL, MDA, or Methylglyoxal modified proteins.

Purification: Protein G Purified

Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).

Clone: 6F10

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• FACS (1:50)
• FCM (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5067282 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 μg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: 4-Hydroxy-2-Hexenal (4-HHE)

Alternative Name: 4-Hydroxy-2-hexenal

Target Type: Chemical

Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.