PD-1 antibody (AA 21-167)

Catalog No. ABIN532977

Product Details anti-PD-1 Antibody

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Binding Specificity: AA 21-167

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purpose: Mouse monoclonal antibody raised against partial recombinant PDCD1.

Cross-Reactivity: Human

Immunogen: Recombinant protein corresponding to amino acids 21-167 of human PDCD1.

Clone: 4F12

Isotype: IgG2b

Application Details

Application Notes: Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS, pH 7.4 (10 % glycerol, 0.02 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PD-1 antibody (ABIN532977)

Latchman, Wood, Chernova, Chaudhary, Borde, Chernova, Iwai, Long, Brown, Nunes, Greenfield, Bourque, Boussiotis, Carter, Carreno, Malenkovich, Nishimura, Okazaki, Honjo, Sharpe, Freeman: "PD-L2 is a second ligand for PD-1 and inhibits T cell activation." in: Nature immunology, Vol. 2, Issue 3, pp. 261-8, (2001) (PubMed).

Nishimura, Honjo: "PD-1: an inhibitory immunoreceptor involved in peripheral tolerance." in: Trends in immunology, Vol. 22, Issue 5, pp. 265-8, (2001) (PubMed).

Okazaki, Maeda, Nishimura, Kurosaki, Honjo: "PD-1 immunoreceptor inhibits B cell receptor-mediated signaling by recruiting src homology 2-domain-containing tyrosine phosphatase 2 to phosphotyrosine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 24, pp. 13866-71, (2001) (PubMed).

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: CD279 / PD1 (PDCD1 Products)

Synonyms: CD279 antibody, PD-1 antibody, PD1 antibody, SLEB2 antibody, hPD-1 antibody, hPD-l antibody, Ly101 antibody, Pdc1 antibody, programmed cell death 1 antibody, PDCD1 antibody, Pdcd1 antibody

Gene ID: 5133

Pathways: Cancer Immune Checkpoints