Histone H3.3 antibody (AA 7-18)

Catalog No. ABIN533007

Product Details anti-Histone H3.3 Antibody

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Binding Specificity: AA 7-18

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Mouse monoclonal antibody raised against synthetic phosphopeptide of H3F3A/H3F3B.

Cross-Reactivity: Human, Mouse

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to amino acids 7-18 residues surrounding S10 of human H3F3A/H3F3B.

Clone: 44AT1232

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Histone H3.3 antibody (ABIN533007)

Wong, McGhie, Sim, Anderson, Ahn, Hannan, George, Morgan, Mann, Choo: "ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells." in: Genome research, Vol. 20, Issue 3, pp. 351-60, (2010) (PubMed).

Elsaesser, Goldberg, Allis: "New functions for an old variant: no substitute for histone H3.3." in: Current opinion in genetics & development, Vol. 20, Issue 2, pp. 110-7, (2010) (PubMed).

Goldberg, Banaszynski, Noh, Lewis, Elsaesser, Stadler, Dewell, Law, Guo, Li, Wen, Chapgier, DeKelver, Miller, Lee, Boydston, Holmes, Gregory, Greally, Rafii, Yang, Scambler, Garrick, Gibbons, Higgs et al.: "Distinct factors control histone variant H3.3 localization at specific genomic regions. ..." in: Cell, Vol. 140, Issue 5, pp. 678-91, (2010) (PubMed).

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3F3A (H3F3A Products)

Synonyms: H3.3A antibody, H3F3 antibody, h3f3a antibody, MGC52829 antibody, wu:fb58e10 antibody, zgc:56193 antibody, zgc:86731 antibody, H3-I antibody, H3-II antibody, H3-III antibody, H3-IV antibody, H3-V antibody, H3-VI antibody, H3-VII antibody, H3-VIII antibody, H3F3A antibody, h3.3 antibody, h3.3a antibody, CG5825 antibody, Dmel\\CG5825 antibody, H3.3 antibody, His-3.3A antibody, His3.3 antibody, PH3 antibody, dH3.3A antibody, H3 histone family member 3A antibody, H3 histone, family 3A antibody, H3 histone, family 3A L homeolog antibody, H3 histone, family 3C antibody, histone H3.3 antibody, histone cluster 1, H3a antibody, Histone H3.3A antibody, H3F3A antibody, h3f3a.L antibody, h3f3a antibody, H3F3C antibody, LOC475916 antibody, LOC480110 antibody, HIST1H3A antibody, LOAG_05159 antibody, His3.3A antibody, H3f3a antibody

Gene ID: 3020, 3021