Spectrin beta Chain, Erythrocyte (SPTB) antibody

Catalog No. ABIN534235

Product Details

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Mouse monoclonal antibody raised against native SPTB.

Specificity: This antibody is specific to the 220kD human erythroid beta-spectrin. The protein is also present in the membranes of human skeletal muscle cells. Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific to erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics. Monoclonal antibody to erythroid beta-spectrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Native Purified Protein.

Immunogen: Native purified human SPTB.

Clone: DB2

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (1 % BSA, 0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

References

Auranen, Rapola, Pihko, Haltia, Leivo, Soinila, Virtanen, Kalimo, Anderson, Santavuori, Somer: "Muscle membrane-skeleton protein changes and histopathological characterization of muscle-eye-brain disease." in: Neuromuscular disorders : NMD, Vol. 10, Issue 1, pp. 16-23, (2000) (PubMed).

Target Details

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Alternative Name: Spectrin beta chain (erythrocyte) / SPTB (SPTB Products)

Synonyms: AI842465 antibody, D330027P03Rik antibody, Gm1301 antibody, Spnb-1 antibody, Spnb1 antibody, ja antibody, jaundiced antibody, mKIAA4219 antibody, EL3 antibody, HS2 antibody, HSPTB1 antibody, SPH2 antibody, spectrin beta, erythrocytic antibody, spectrin, beta, erythrocytic antibody, SPTB antibody, Sptb antibody

Gene ID: 6710

Pathways: Regulation of Actin Filament Polymerization