CD235a/GYPA antibody (N-Term) (FITC)

Catalog No. ABIN535966

Product Details anti-CD235a/GYPA Antibody (FITC)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Aggregation (AGG)

Purpose: Mouse monoclonal antibody raised against synthetic peptide of GYPA.

Specificity: This antibody recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Cross-Reactivity: Human

Immunogen: A synthetic peptide corresponding to N-terminus of human GYPA.

Clone: HIR2

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 10⁶ cells in a suspension)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.2 % BSA, 0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.

References for anti-CD235a/GYPA antibody (FITC) (ABIN535966)

Bloemen, Wu, Devreese, de Laat, Rand, Vasovic: "Inverted erythrocyte membranes demonstrate β2GPI-antiphospholipid antibody interactions and membrane crosslinking." in: Thrombosis research, Vol. 146, pp. 89-94, (2016) (PubMed).

Yajima, Urano-Tashiro, Shimazu, Takashima, Takahashi, Konishi: "Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane." in: Microbiology and immunology, Vol. 52, Issue 2, pp. 69-77, (2008) (PubMed).

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a / GYPA (GYPA Products)

Synonyms: GYPA antibody, gpa antibody, AI853584 antibody, CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, HGpMiV antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, glycophorin A (MNS blood group) antibody, glycophorin A antibody, GYPA antibody, Gypa antibody

Gene ID: 2993

Pathways: Maintenance of Protein Location