ASAP1 antibody (AA 777-787)

Catalog No. ABIN538233

Product Details anti-ASAP1 Antibody

Target: ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))

Binding Specificity: AA 777-787

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of Asap1.

Specificity: Reactivity occurs against both the phosphorylated and non-phosphorylated forms of the protein at residue Y782.

Cross-Reactivity: Chicken, Chimpanzee, Cow, Dog, Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: A synthetic peptide corresponding to amino acids 777-787 of mouse Asap1.

Application Details

Application Notes: ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-ASAP1 antibody (ABIN538233)

Liu, Yerushalmi, Grigera, Parsons: "Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling." in: The Journal of biological chemistry, Vol. 280, Issue 10, pp. 8884-92, (2005) (PubMed).

Kruljac-Letunic, Moelleken, Kallin, Wieland, Blaukat: "The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29560-70, (2003) (PubMed).

Liu, Loijens, Martin, Karginov, Parsons: "The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly." in: Molecular biology of the cell, Vol. 13, Issue 6, pp. 2147-56, (2002) (PubMed).

Target Details for ASAP1

Target: ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))

Alternative Name: ASAP1 / DDEF1 (ASAP1 Products)

Synonyms: AV239055 antibody, DEF-1 antibody, Ddef1 antibody, PAP antibody, mKIAA1249 antibody, s19 antibody, AMAP1 antibody, CENTB4 antibody, DDEF1 antibody, PAG2 antibody, ZG14P antibody, ASAP1 antibody, ddef1 antibody, Asap1 antibody, si:ch211-160i2.3 antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain1 antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 antibody, toxic extracellular endopeptidase antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 L homeolog antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a antibody, Asap1 antibody, ASAP1 antibody, asaP1 antibody, asap1 antibody, asap1.L antibody, asap1a antibody

Gene ID: 13196