ELISA (1:4000-1:9000) Western Blot (1:1000-1:15000) Immunoprecipitation (0.8-1.5 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Zhou, Dewille: "Proteasome-mediated CCAAT/enhancer-binding protein delta (C/EBPdelta) degradation is ubiquitin-independent." in: The Biochemical journal, Vol. 405, Issue 2, pp. 341-9, (2007) (PubMed).
Shin, Jeon, Yang, Her, Kim, Kim, Kim: "CCAAT/enhancer-binding protein delta activates the Runx2-mediated transcription of mouse osteocalcin II promoter." in: Journal of molecular endocrinology, Vol. 36, Issue 3, pp. 531-46, (2006) (PubMed).
Thangaraju, Ananth, Martin, Roon, Smith, Sterneck, Prasad, Ganapathy: "c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney." in: The Journal of biological chemistry, Vol. 281, Issue 37, pp. 26769-73, (2006) (PubMed).
Target
CEBPD
(CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))