RFFL antibody (AA 1-363)

Catalog No. ABIN539474

Product Details anti-RFFL Antibody

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Binding Specificity: AA 1-363

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against partial recombinant RFFL.

Specificity: This protein A purified antibody is directed against human RFFL protein. Expect reactivity with isoform 1, 2 and 3 of RFFL.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: Recombinant protein corresponding to amino acids 1-363 of human RFFL.

Application Details

Application Notes: ELISA (1:4000-1:20000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.02 M KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-RFFL antibody (ABIN539474)

McDonald, El-Deiry: "Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 16, pp. 6170-5, (2004) (PubMed).

Coumailleau, Das, Alcover, Raposo, Vandormael-Pournin, Le Bras, Baldacci, Dautry-Varsat, Babinet, Cohen-Tannoudji: "Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment." in: Molecular biology of the cell, Vol. 15, Issue 10, pp. 4444-56, (2004) (PubMed).

Tibbetts, Shiozaki, Gu, McDonald, El-Deiry, Shi: "Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2." in: Structure (London, England : 1993), Vol. 12, Issue 12, pp. 2257-63, (2004) (PubMed).

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL / RNF189 (RFFL Products)

Synonyms: MGC84042 antibody, RFFL antibody, Rffl antibody, 1700051E09Rik antibody, 4930516L10Rik antibody, BG080975 antibody, Carp2 antibody, CARP-2 antibody, CARP2 antibody, FRING antibody, RIFIFYLIN antibody, RNF189 antibody, RNF34L antibody, ring finger and FYVE like domain containing E3 ubiquitin protein ligase antibody, ring finger and FYVE-like domain containing E3 ubiquitin protein ligase L homeolog antibody, ring finger and FYVE-like domain containing 1 antibody, ring finger and FYVE like domain containing protein antibody, ring finger and FYVE-like domain containing E3 ubiquitin protein ligase antibody, RFFL antibody, rffl.L antibody, Rffl antibody

Gene ID: 117584