Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
Purpose
Rabbit polyclonal antibody raised against synthetic phosphopeptide of LSP1.
Specificity
This antibody recognizes ~52 KDa of human LSP1 at a phosphorylated serine 252. It does not recognize non-phosphorylated peptides confirmed by dot blot.
Cross-Reactivity
Human, Mouse
Characteristics
Antibody Reactive Against Synthetic Peptide.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S252 of human LSP1.
LSP1
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot (0.1-1 μg/mL) ELISA (0.01-0.1 μg/mL) Immunoprecipitation (2-5 μg/mL) Immunohistochemistry (2-5 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In TBS, pH 7.2 (BSA, 10 % Proclin300)
Preservative
ProClin
Precaution of Use
This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C,-80 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
Liu, Cara, Kaur, Raharjo, Mullaly, Jongstra-Bilen, Jongstra, Kubes: "LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration." in: The Journal of experimental medicine, Vol. 201, Issue 3, pp. 409-18, (2005) (PubMed).