ROCK2 antibody (pThr396)

Catalog No. ABIN540951

Product Details anti-ROCK2 Antibody

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Binding Specificity: pThr396

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of ROCK2.

Sequence: VETFP

Specificity: This antibody recognizes ~165 KDa of human ROCK2 at a phosphorylation site of Thr396. It does not react to non-phosphorylated protein.

Cross-Reactivity: Chicken, Cow, Dog, Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T396 of human ROCK2.

Application Details

Application Notes: Western Blot (0.1-1 μg/mL)
ELISA (0.01-0.1 μg/mL)
Immunoprecipitation (2-5 μg/mL)
Immunohistochemistry (0.5-2 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In TBS, pH 7.2 (BSA, 10 % Proclin300)

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.

References for anti-ROCK2 antibody (ABIN540951)

Vahebi, Kobayashi, Warren, de Tombe, Solaro: "Functional effects of rho-kinase-dependent phosphorylation of specific sites on cardiac troponin." in: Circulation research, Vol. 96, Issue 7, pp. 740-7, (2005) (PubMed).

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: ROCK2 (ROCK2 Products)

Synonyms: rocka-a antibody, xROK antibody, rock2 antibody, rok2 antibody, ROCK2 antibody, B230113H15Rik antibody, ROKalpha antibody, Rho-kinase antibody, Rock-II antibody, Rock2m antibody, mKIAA0619 antibody, ROCK-II antibody, ROK antibody, Rho-associated, coiled-coil containing protein kinase 2 L homeolog antibody, rho-associated, coiled-coil containing protein kinase 2a antibody, Rho associated coiled-coil containing protein kinase 2 antibody, Rho-associated, coiled-coil containing protein kinase 2 antibody, Rho-associated coiled-coil containing protein kinase 2 antibody, rock2.L antibody, rock2a antibody, ROCK2 antibody, Rock2 antibody

Gene ID: 9475

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation