NEK2 antibody (Internal Region)

Catalog No. ABIN544242

Product Details anti-NEK2 Antibody

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of NEK2.

Cross-Reactivity: Human, Mouse

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to internal region of human NEK2.

Application Details

Application Notes: Immunofluorescence (1:25)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-NEK2 antibody (ABIN544242)

Chen, Riley, Zheng, Chen, Lee: "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49408-16, (2002) (PubMed).

Eto, Elliott, Prickett, Brautigan: "Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44013-20, (2002) (PubMed).

Schutte, Bjork, Coppage, Malik, Gregory, Scott, Brentzell, Watanabe, Dixon, Murray: "A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41." in: Genome research, Vol. 10, Issue 1, pp. 81-94, (2000) (PubMed).

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2 (NEK2 Products)

Synonyms: AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody

Gene ID: 4751

Pathways: M Phase