Integrin beta 4 antibody (pTyr1494)

Catalog No. ABIN548462

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Binding Specificity: pTyr1494

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of ITGB4.

Specificity: This peptide sequence is found in all three integrin-b4 isoforms and is highly conserved in rat and mouse integrin-b4.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1494 of human ITGB4.

Application Details

Application Notes: ELISA (1:4000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Integrin beta 4 antibody (ABIN548462)

Kim, Huang, Cheepala, Huang, Chung: "Curcumin inhibition of integrin (alpha6beta4)-dependent breast cancer cell motility and invasion." in: Cancer prevention research (Philadelphia, Pa.), Vol. 1, Issue 5, pp. 385-91, (2009) (PubMed).

Shaw: "Identification of insulin receptor substrate 1 (IRS-1) and IRS-2 as signaling intermediates in the alpha6beta4 integrin-dependent activation of phosphoinositide 3-OH kinase and promotion of invasion." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5082-93, (2001) (PubMed).

Dans, Gagnoux-Palacios, Blaikie, Klein, Mariotti, Giancotti: "Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1494-502, (2001) (PubMed).

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104 (ITGB4 Products)

Synonyms: AA407042 antibody, C230078O20 antibody, CD104 antibody, integrin subunit beta 4 antibody, integrin beta 4 antibody, ITGB4 antibody, Itgb4 antibody

Gene ID: 3691

Pathways: Integrin Complex