LIG1 antibody

Catalog No. ABIN2624893

The Mouse Monoclonal anti-LIG1 antibody (Clone 10H5) (ABIN2624893) specifically detects LIG1 in WB, IHC, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for LIG1 antibody (ABIN2624893)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 10H5

Product Details anti-LIG1 Antibody

Specificity: Full-length recombinant human DNA Ligase I protein

Purification: Protein G purified

Immunogen: Recombinant human LIG1.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2. No preservatives added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1 / DNA Ligase 1

Background: Name/Gene ID: LIG1

Synonyms: LIG1, DNA ligase I, DNA ligase 1, Ligase I, DNA, ATP-dependent

Gene ID: 3978

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA