UBTF antibody (C-Term)

Catalog No. ABIN5515000

The Rabbit Polyclonal anti-UBTF antibody is suitable to detect UBTF in samples from Human, Rat, Mouse, Horse, Cow, Dog, Rabbit, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for UBTF antibody (C-Term) (ABIN5515000)

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Reactivity: Human, Rat, Mouse, Horse, Cow, Dog, Rabbit, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBTF Antibody

Binding Specificity: C-Term

Purpose: UBTF Antibody - C-terminal region

Sequence: SEDGGDSSES SSEDESEDGD ENEEDDEDED DDEDDDEDED NESEGSSSSS

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 86%, Rabbit: 93%, Rat: 93%, Yeast: 86%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against UBF1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human UBF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39068-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBTF

Background:

Background Information: This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11.

Gene Name: upstream binding transcription factor

Alternative Symbols: UBF, UBF1, UBF2, UBF-1, CONDBA, NOR-90

Protein Name: nucleolar transcription factor 1

Molecular Weight: 84kDa

Gene ID: 7343

NCBI Accession: XP_006722124

UniProt: P17480