JUP antibody (N-Term)

Catalog No. ABIN5518649

The Rabbit Polyclonal anti-JUP antibody (ABIN5518649) specifically detects JUP in WB, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for JUP antibody (N-Term) (ABIN5518649)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-JUP Antibody

Binding Specificity: N-Term

Purpose: Anti-gamma Catenin/JUP Antibody

Sequence: LLATQVEGQA TNLQRLAE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by one amino acid.

Characteristics: Anti-gamma Catenin/JUP Antibody-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human gamma Catenin, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Background:

Background: Cateningamma, also known as junction plakoglobin (JUP) or plakoglobin (PKGB). Plakoglobin is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. Cateninbeta and cateningamma (plakoglobin), vertebrate homologs of Drosophila armadillo, function in cell adhesion and the Wnt signaling pathway. Cateningamma may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.

Gene Full Name: junction plakoglobin

Sequence Similarities: Belongs to the beta-catenin family.

Molecular Weight: 82 kDa

Gene ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location