CD80 antibody (C-Term)

Catalog No. ABIN5518665

This Rabbit Polyclonal antibody specifically detects CD80 in WB. It exhibits reactivity toward Rat.It has been mentioned in 1 publication

Quick Overview for CD80 antibody (C-Term) (ABIN5518665)

Target: CD80

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD80 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD80 Antibody

Binding Specificity: C-Term

Purpose: Anti-CD80 Antibody

Sequence: DAHVSQNFTW EKPPEDPPD

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by two amino acids.

Characteristics: Anti-CD80 Antibody-1. Tested in WB applications. This antibody reacts with Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD80 antibody (ABIN5518665)

Du, Dong, Zhao, Fu, Wang, Chen, Ou, Li, Sun, Tang, Song: "Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice." in: Acta pharmacologica Sinica, Vol. 33, Issue 8, pp. 1047-54, (2012) (PubMed).

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background:

Background: Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al. (1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Gene Full Name: CD80 Molecule

Molecular Weight: 50 kDa

UniProt: O55202

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints