VEGFR2/CD309 antibody (AA 20-242)

Catalog No. ABIN5519021

This anti-VEGFR2/CD309 antibody is a Rabbit Polyclonal antibody detecting VEGFR2/CD309 in WB, IHC, FACS and ICC. Suitable for Human. This Primary Antibody has been cited in 4+ publications.

Quick Overview for VEGFR2/CD309 antibody (AA 20-242) (ABIN5519021)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFR2/CD309 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-VEGFR2/CD309 Antibody

Binding Specificity: AA 20-242

Purpose: Anti-VEGF Receptor 2/KDR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-VEGF Receptor 2/KDR Antibody Picoband® (ABIN5519021). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human VEGF Receptor 2 recombinant protein (Position: A20-L242).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
ELISA (Cap),1-5 μg/mL
1. Matthews, W., Jordan, C. T., Gavin, M., Jenkins, N. A., Copeland, N. G., Lemischka, I. R. A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit. Proc. Nat. Acad. Sci. 88: 9026-9030, 1991. 2. Plate, K. H., Breier, G., Millauer, B., Ullrich, A., Risau, W. Up-regulation of vascular endothelial growth factor and its cognate receptors in a rat glioma model of tumor angiogenesis. Cancer Res. 53: 5822-5827, 1993. 3. Sait, S. N. J., Dougher-Vermazen, M., Shows, T. B., Terman, B. I. The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-q12. Cytogenet. Cell Genet. 70: 145-146, 1995.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-VEGFR2/CD309 antibody (ABIN5519021)

Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).

Zhu, Tuerxun, Hui, Abliz: "Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 647-651, (2014) (PubMed).

Liu, Yang, Zhang, Shui, Song, Yao, Dai, Sun: "Fructopyrano-(1?4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 3859-69, (2014) (PubMed).

Wu, You, Ma, Li, Yuan, Li, Ye, Liu, Yao, Chen, Lai, Yang: "Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice." in: PLoS ONE, Vol. 8, Issue 5, pp. e62827, (2013) (PubMed).

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: KDR

Background:

Synonyms: Vascular endothelial growth factor receptor 2, VEGFR-2, 2.7.10.1, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD309, KDR, FLK1, VEGFR2,

Tissue Specificity: Detected in cornea (at protein level). Widely expressed.

Background: KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells. Flk1 is the mouse homolog of KDR.

Molecular Weight: 180 kDa

Gene ID: 3791

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling