Aurora A antibody (N-Term)

Catalog No. ABIN5530245

The Rabbit Polyclonal anti-Aurora A antibody has been validated for WB. It is suitable to detect Aurora A in samples from Mouse.

Quick Overview for Aurora A antibody (N-Term) (ABIN5530245)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aurora A Antibody

Binding Specificity: AA 93-120, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Aurka antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of mouse Aurka.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurka

Background: Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

Molecular Weight: 45 kDa

Gene ID: 20878

UniProt: P97477

Pathways: Cell Division Cycle, Asymmetric Protein Localization