Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This NCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK2.
NCK2
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
GRB4 antibody, NCKbeta antibody, NCK2 antibody, 4833426I10Rik antibody, Grb4 antibody, Nck-2 antibody, Nck2 antibody, zgc:55283 antibody, fd20h08 antibody, im:7147817 antibody, si:ch211-147g19.3 antibody, wu:fd20h08 antibody, zgc:92024 antibody, NCK adaptor protein 2 antibody, cytoplasmic protein NCK2-like antibody, non-catalytic region of tyrosine kinase adaptor protein 2 antibody, NCK adaptor protein 2a antibody, NCK adaptor protein 2b antibody, NCK2 antibody, Nck2 antibody, LOC100227348 antibody, nck2a antibody, nck2b antibody
Background
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.