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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 260-289), (C-Term) antibody Primary Antibody

NCF4 Reactivity: Human FACS, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5537261
$570.63
Plus shipping costs $45.00
400 μL
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  • Target
    Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
    Binding Specificity
    AA 260-289, C-Term
    • 17
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Human
    • 85
    • 56
    • 41
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    • 44
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Application
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    • 49
    • 26
    • 22
    • 19
    • 11
    • 5
    • 2
    • 2
    Purification
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
    Immunogen
    This NCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human NCF4.
    Isotype
    Ig Fraction
  • Application Notes
    For WB starting dilution is: 1:1000

    For IHC-P starting dilution is: 1:50~100

    For FACS starting dilution is: 1:10~50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2 mg/mL
    Buffer
    Supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
    Alternative Name
    NCF4 (NCF4 Antibody Abstract)
    Synonyms
    p40phox, AI451400, NCF, P40PHOX, SH3PXD4, neutrophil cytosolic factor 4, ncf4, Ncf4, NCF4
    Background
    NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2(NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity.
    Molecular Weight
    39 kDa
    Gene ID
    4689
    UniProt
    Q15080
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