ZAP70 antibody (N-Term)

Catalog No. ABIN5537406

The Rabbit Polyclonal anti-ZAP70 antibody is suitable to detect ZAP70 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for ZAP70 antibody (N-Term) (ABIN5537406)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZAP70 Antibody

Binding Specificity: AA 103-130, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human ZAP70.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Molecular Weight: 70 kDa

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway