CRTC2 antibody (C-Term)

Catalog No. ABIN5539499

This Goat Polyclonal antibody specifically detects CRTC2 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for CRTC2 antibody (C-Term) (ABIN5539499)

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CRTC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-CRTC2 Antibody

Binding Specificity: C-Term

Purpose: CRTC2

Sequence: DPAVEDSFRS DRLQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa observed in lysates of cell line Jurkat and in preliminary testing of NIH3T3, and approx 85 kDa in Rat Brain lysates and lysates of cell line A431 (calculated MW of 73.2 kDa according to Human, NP_083157.1) and Rat NP_001029067

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CRTC2

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Alternative Name: CRTC2

Background: Crtc2, CREB regulated transcription coactivator 2, 4632407F12Rik, Torc2, OTTMUSP00000023505, transducer of regulated cAMP response element-binding protein (CREB) 2

Gene ID: 200186, 74343, 310615

NCBI Accession: NP_083157

Pathways: AMPK Signaling, Carbohydrate Homeostasis