alpha Adaptin antibody (AA 706-727)

Catalog No. ABIN5539681

Cited in 1 publication. The Goat Polyclonal anti-alpha Adaptin antibody (ABIN5539681) specifically detects alpha Adaptin in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for alpha Adaptin antibody (AA 706-727) (ABIN5539681)

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Adaptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-alpha Adaptin Antibody

Binding Specificity: AA 706-727

Purpose: AP2A1 (aa706-727)

Sequence: ELEPPAPESP MALLADPAPA AD

Specificity: This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108 kDa according to NP_055018.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-alpha Adaptin antibody (ABIN5539681)

Buser, Ritz, Moes, Tostado, Frank, Spiess, Mariani, Jenö, Boulay, Hutter: "Quantitative proteomics reveals reduction of endocytic machinery components in gliomas." in: EBioMedicine, Vol. 46, pp. 32-41, (2020) (PubMed).

Target Details for alpha Adaptin

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Alternative Name: AP2A1

Background: AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit

Gene ID: 160, 11771, 308578

NCBI Accession: NP_055018

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation