GRB2 antibody (C-Term) (Biotin)

Catalog No. ABIN5539730

This Goat Polyclonal antibody specifically detects GRB2 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for GRB2 antibody (C-Term) (Biotin) (ABIN5539730)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GRB2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GRB2 Antibody (Biotin)

Binding Specificity: C-Term

Purpose: GRB2, biotinylated

Sequence: PRNYVTPVNR NV.

Specificity: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in lysates of Human Thymus and of cell line MOLT4 (calculated MW of 25.2 kDa according to NP_002077.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2

Background: GRB2, growth factor receptor-bound protein 2, ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2, HT027, SH2/SH3 adapter GRB2, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, protein Ash

Gene ID: 2885, 14784, 81504

NCBI Accession: NP_002077, NP_987102

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation