NSG2 antibody (C-Term)

Catalog No. ABIN5539930

This anti-NSG2 antibody is a Goat Polyclonal antibody detecting NSG2 in ELISA, WB and ICC. Suitable for Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NSG2 antibody (C-Term) (ABIN5539930)

Target: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NSG2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-NSG2 Antibody

Binding Specificity: C-Term

Purpose: Nsg2 (mouse, C terminus)

Sequence: HEPKPPKTQG H

Specificity: Reported variants represent identical protein: NP_001277610.1, NP_001277609.1, NP_032767.1

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 19 kDa band observed in Mouse fetal Brain lysates (calculated MW of 19.0 kDa according to NP_032767.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Rat: Chan

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunocytochemistry: This antibody has been successfully used in ICC on Rat: Chan Choo Yap et al. (2022) PMID: 35474277.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NSG2 antibody (ABIN5539930)

Yap, Digilio, McMahon, Garcia, Winckler: "Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes." in: The Journal of cell biology, Vol. 217, Issue 9, pp. 3141-3159, (2019) (PubMed).

Yap, Digilio, McMahon, Winckler: "The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes." in: Scientific reports, Vol. 7, Issue 1, pp. 10481, (2019) (PubMed).

Target Details for NSG2

Target: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Alternative Name: Nsg2

Background: Nsg2, neuron specific gene family member 2, protein 8.5, protein p19

Gene ID: 18197

NCBI Accession: NP_032767