Clone GM1C8 recognises IL-13Rα1 transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on monocytes (W. Luttmann, unpublished data). Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells. Species: Human.
Purification
Protein G chromatography
Immunogen
cDNA of the human IL-13Ralpha1 chain extracellular domain (U62858).
IL13RA1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1F5
unconjugated
Application Notes
Flow cytometry: 1.2 μg/10 6 cells. The antibody is routinely tested by on BOSC cells transiently transfected with an IL-13Rα1 expression vector. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. It is expected that the antibody will be suited for all applications where recognition of the native protein is required.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.2 Preservatives: 0.01 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Kunii, Nemoto, Kanaya, Tsubahara, Shimauchi: "Expression of CD13/aminopeptidase N on human gingival fibroblasts and up-regulation upon stimulation with interleukin-4 and interleukin-13." in: Journal of periodontal research, Vol. 40, Issue 2, pp. 138-46, (2005) (PubMed).
Bade, Lohrmann, ten Brinke, Wolbink, Wolbink, ten Berge, Virchow, Luttmann, Hack: "Detection of soluble human granzyme K in vitro and in vivo." in: European journal of immunology, Vol. 35, Issue 10, pp. 2940-8, (2005) (PubMed).
Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and im-mune responses. IL-13Rα1 together with IL-4Rα forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.