Collagen IV antibody

Catalog No. ABIN5541082

This anti-Collagen IV antibody is a Mouse Monoclonal antibody detecting Collagen IV in WB, IHC (fro), IHC (p), FACS, IF and ICC. Suitable for Human, Pig and Cow.

Quick Overview for Collagen IV antibody (ABIN5541082)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Pig, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1043

Product Details anti-Collagen IV Antibody

Specificity: This antibody clone 1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological tissues.

Cross-Reactivity (Details): Does NOT react with Rat and Rabbit.

Purification: Purified

Immunogen: Purified Human type IV Collagen, isolated from Human placenta. Antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.

Isotype: IgG1

Application Details

Application Notes: Western blot : 1/100-1/1000. Immunocytochemistry on fixed cells (methanol fixation). Flow cytometry. Immunohistochemistry on Frozen Sections using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl. Immunohistochemistry on Paraffin Sections : Pretreatement with 1 mg/mL pepsin in 0.01N HCl for 30 minutes at room temperature is recommended. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: collagen type iv

Background: Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology.

UniProt: P02462