CD55 antibody

Catalog No. ABIN5541173

Product Details anti-CD55 Antibody

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: The monoclonal antibody PD2 recognizes Pig complement decay accelerating factor (DAF), also designated as CD55.

Purification: Purified

Immunogen: Pig DAF-Ig fusion protein.

Clone: PD2

Isotype: IgG1

Application Details

Application Notes: Immuno Assays. Flow Cytometry (Ref.2). Western blot. Positive Control: Porcine platelet lysate. Typical starting working dilution: 1/50.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Stabilizers: 0.1 % BSA

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: cd55,daf (CD55 Products)

Synonyms: CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody

Background: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Pig DAF (CD55) is a 45-52 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. DAF is broadly distributed among cells in contact with serum, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complementmediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity