NMDAR2A antibody (C-Term)

Catalog No. ABIN5541473

This Rabbit Polyclonal antibody specifically detects NMDAR2A in WB and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NMDAR2A antibody (C-Term) (ABIN5541473)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDAR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-NMDAR2A Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~180k NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Purification: Affinity purification using a column to which the fusion protein immunogen was coupled

Immunogen: Fusion protein from the C-terminus of the NR2A subunit of rat NMDA receptor.

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: Immunoprecipitation: 6 μL per 200 μg lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Alternative Name: nmda receptor 2a

Background: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K /AMPAR). Those that are sensitive to N-methyl-D- aspartate (NMDA) are designated NMDA receptors (N MDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been im plicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002 Wenthold et al., 2003 Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989 Alvestad et al., 2003 Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such cha nnels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity