GFP antibody

Catalog No. ABIN5541514

This anti-GFP antibody is a Chicken Polyclonal antibody detecting GFP in WB, IF, ICC and IHC (fro). Suitable for Aequorea victoria.

Quick Overview for GFP antibody (ABIN5541514)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GFP Antibody

Specificity: Recognizes Green Fluorescent Protein (GFP). Quality Control: Antibodies were analyzed by Western blot analysis (1/5000) and Immunohistochemistry (1/500) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® as the blocking reagent, and HRP-labeled Goat anti-Chicken antibody (Cat.-No AP31795HR-N) as the detection reagent. Immunohistochemistry used Tetramethyl Rhodamine-Labeled anti-Chicken IgY.

Purification: Affinity Chromatography

Immunogen: Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot (1/2500-1/5000). Immuofluorescence. Immunohistochemistry (1/1000).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Sodium Phosphate-Buffered (10 mM, pH 7.2) isotonic (0.9 % , w/v) Saline with 50 % (v/v) Glycerol as an anti-freezing agent and 0.01 % (w/v) Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20°C in the dark. Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: gfp

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

UniProt: P42212